代表中国斑马鱼研究最高水平之一的全国斑马鱼研究大会9月7日在青岛成功举办。
金年会仪器公司作为金牌赞助商参加会议。
VAST BioImager是美国Union Biometrica公司开发的专用于斑马鱼幼鱼的高通量图像采集系统,金年会仪器做为美国Union Biometrica公司中国地区的授权代理商,携样机在“第四届全国斑马鱼研究大会”成功展出,吸引了众多与会者的极大关注。VAST BioImager斑马鱼图像采集系统这一先进技术,获得会议上很多知名专家的称赞。
VAST bioImager斑马鱼图像采集系统,专门为2-7天斑马鱼幼体设计。在采集图像之前自动完成斑马鱼幼体装载、定位、旋转等操作,避免了在进行高通量图像采集时对每条斑马鱼幼体人工操作的时间损耗和冗长的步骤。
VAST bioImager斑马鱼图像采集系统可安装在多种直立复式显微镜或者立体显微镜上进行高分辨率细胞水平上的图像采集和荧光信号的观察。
VAST bioImager斑马鱼图像采集系统产品特点:
斑马鱼全身器官水平成像
·自动处理图像采集前斑马鱼幼体的各种操作
·避免人工操作的时间损耗和冗长的步骤
·在软件上点击“Load one Fish”,VAST就会装载好一个斑马鱼幼体
·系统可将斑马鱼幼体进行360°旋转进行定 位操作者可根据自己的意愿选择方向,侧面、背面或者其他方向进行图像采集
斑马鱼高分辨率细胞水平成像,荧光成像
·适用于高通量&高内涵筛选
·可安装在多种直立复式显微镜或者立体显微镜上
·可安装在荧光显微镜上进行荧光图像采集
·可选配的LP采样器,直接从多孔板中传送斑马鱼幼体到VAST进行图像采集
For higher resolution imaging including cellular level imaging tasks for high content screens, VAST can be mounted on many models of upright compound microscopes or stereomicroscopes using the optional High Resolution Microscope Stage and Mounting Kit. Please see our specification sheet for critical microscope dimensions. (Note: Inverted microscopes can NOT be used.) The stage in this optional kit replaces the original stage found on select upright microscopes and enables automated micron-level positioning laterally along the zebrafish.
When using VAST with a microscope, once the larva is positioned using the on-board camera, the VAST internal light source is turned off and high-resolution images can be captured with the microscope's camera. Mounting VAST on a microscope equipped with fluorescence capability allows collection of fluorescence images.
The optional External Communications Package includes TTL inputs & outputs for automating communications with most microscopes and cameras.
可选配斑马鱼微孔板自动进样系统
The optional LP Sampler can be added to the system to gently aspirate larvae from multi-well plates and deliver them one at a time to VAST for imaging. This allows for increased throughput of high content assays and screens
VAST BioImager斑马鱼图像采集系统技术参数
相机:系统相机,可在明场下采集斑马鱼个体水平图像,
相机分辨率: 10 μm
旋转定位精确度:± 3°(野生型斑马鱼)
横向定位精确度:±2 mm(野生型斑马鱼)
±100 um (野生型斑马鱼),高分辨率平台(选配)
斑马鱼处理: 使用镇静剂,2-7dpf(days post fertilization)的幼鱼
分选功能:可以将斑马鱼幼体分流到两个不同的接收容器中
显微镜类型:正置复合光显微镜或立体显微镜
VAST BioImager斑马鱼图像采集系统相关文献
· High-throughput hyperdimensional vertebrate phenotyping.
Carlos Pardo-Martin, et.al., Nature Communications, Volume:4, Article
number:1467, DOI:doi:10.1038/ncomms2475
· Presenting VAST BioImager™: A new modular,expandable platform to automate
the orientation of 2-7 day old zebrafish larvae for imaging.
Union Biometrica, Geel, Belgium, Union Biometrica, Holliston, MA, USA
Yanik lab, MIT, Boston, MA, USA
· Fully automated cellular-resolution vertebrate screening platform with parallel
animal processing.
Chang TY, Pardo-Martin C, Allalou A, Wählby C, Yanik MF.,Lab Chip. 2012 Feb
21;12(4):711-6. doi: 10.1039/c1lc20849g.
· High-throughput in vivo vertebrate screening.
Pardo-Martin C, Chang TY, Koo BK, Gilleland CL, Wasserman SC, Yanik MF.,Nat
Methods. 2010 Aug;7(8):634-6. doi: 10.1038/nmeth.1481.
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